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KMID : 0389420100180030245
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Korean Journal of Stress Research
2010 Volume.18 No. 3 p.245 ~ p.253
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Role of Signal Transducer and Activator of Transcription 6 (Stat6) in Alcohol-induced Brain Damage
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Park Hae-Jeong
Kim Jong-Woo
Cho Ah-Rang
Park Jin-Kyung
Kang Won-Sub
Yoon Doh-Joon
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Abstract
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Signal transducer and activator of transcription 6 (Stat6) modified cell differentiation and growth, and induced the resistance to apoptosis in various tissues and cells. In this study, we assessed the role of Stat6 on acute ethanol-induced intoxication in mouse brain using Stat6 knockout (KO) mouse. The acute ethanol intoxication was induced by an intraperitoneal injection of 20% ethanol (2 g/kg) in BALB/c wild type (WT), and Stat6 KO mice. After collecting the brains of WT and KO mice, we performed 7.4 K mouse cDNA microarray analysis. The expression profiles were compared between non-treated and ethanol-treated groups for WT and Stat6 KO mice respectively. In the brains of WT mice, the changes of the expressions of transcription factor genes related with cell proliferation were shown. The ethanol increased the level of SRY-box containing gene 4 (Sox4), and decreased the level of hairy and enhancer of split 5 (Hes5). In the brains of Stat6 KO mice, the acute ethanol altered the expressions of cell death-related genes. Particularly, the acute ethanol upregulated the expressions of apoptotic genes such as caspase 7 (Casp7) and inositol polyphosphate-5-phosphatase D (Inpp5d), and of cell cycle regulatory factor gene Cyclin-dependent kinase inhibitor 1C (P57) (Cdkn1c). In contrast, it downregulated the expression of the anti-apoptotic gene myelocytomatosis oncogene (Myc). These results indicated that the acute ethanol treatment induced the brain damage, and the deficiency of Stat6 elevated this brain damage via apoptotic pathway. Our study suggests that Stat6 may play a crucial role in ethanol-induced brain damage.
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KEYWORD
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Signal transducer and activator of transcription 6, cDNA microarray, Ethanol, Apoptosis, Brain damage
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